Floating C3 Spinach Discs Lab Concerning Photosynthesis that Determined how Light Intensity Affects the Net Rate of Photosynthesis By Senegar & Mitchell and written by Sandra Monreal Abstract Researchers realize that spinach discs float normally so when infiltrating these discs Calculate the change in volume by subtracting the later gas volume from the earlier gas volume. Include a brief statement describing why you selected the graphing method you did. 3. Background Information: Chloroplasts are located in the cells in the center of a leaf, called the mesophyll, or “middle tissue”. Write a detailed conclusion based on your results. Fill in the table below with the results from your first experiment. What Was The Name Of The Plant You Used To Study Photosynthesis? “The data show …” a. Experiment 1: Measuring the Rate of Photosynthesis For the first experiment, what was the change in volume from 00:01:30 to 00:02:00? In this virtual photosynthesis lab, students can manipulate the light intensity, light color, and distance from the light source. Be sure to clearly indicate the x and y axes, the units used, and the graph title(s). Photosynthesis I: An Assay Utilizing Leaf Disks. A plant is shown in a beaker and test tube which bubbles to indicate the rate of photosynthesis. The FLDA actually measures the rate of photosynthetic oxygen production minus the rate of respiratory oxygen use during the same period. In Volume For 5 Minutes Is 0.4 ML For Example, If The Volume At 5 Minutes Is 4 ML. Answer the lab question – What factors affect the rate of photosynthesis in plants? Question: Photosynthesis SHORT ANSWER Experiment 1: Measuring The Rate Of Photosynthesis Lab Results 1. Experiment 3: Measuring the Rate of Photosynthesis with Floating Leaf Discs. Photosynthesis SHORT ANSWER Experiment 1: Measuring the Rate of Photosynthesis Lab Results 1. Calculate the change in volume for each measurement time. Present your results in a graph. Since cellular respiration is taking place at the same time, consuming oxygen, the rate that the disks rise is an indirect measurement of the net rate of photosynthesis. Compare experimental results to control groups! Measuring the Rate of Photosynthesis; In this section, students learn how to use the floating leaf disk method to measure the rate of net photosynthesis (i.e. ... Like all enzyme-driven reactions the rate of photosynthesis can be measured by either. Start studying Photosynthesis lab. elodea 2. The rate that the disks sink is an indirect measure of the rate of cellular respiration. Students can measure the rate over time. 5. One problem in measuring a rate of photosynthesis is that there is a competing process occurring at the same time, cellular respiration, a process that uses oxygen. 4. Did the rate of photosynthesis increase or decrease due to the independent variable? “The hypothesis was…” a. Steucek, Guy L. Robert J. Hill and Class/Summer 1982. The b. As photosynthesis proceeds oxygen is released into the interior of the leaf which changes the buoyancy--causing the disks to rise. Post-Lab Questions. Fill In The Table Below With The Results From Your First Experiment. Materials: Sodium bicarbonate (Baking soda) ... What is an indirect measurement of the net rate of photosynthesis occurring in the leaf tissue? Lab 8: Determining the Rate of Photosynthesis in a Leaf. 1985. What was the name of the plant you used to study photosynthesis? El Odea Branch 2. Print and Web Resources: ! the rate of photosynthesis minus the rate of cellular respiration). Learn vocabulary, terms, and more with flashcards, games, and other study tools. Can you think of a way to how you might measure the gross rate of photosynthesis with this technique? 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